Recorded Lecture Sets
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2007 conferences
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2006 conferences
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2005 conferences
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1999-2004 conferences
Please click here for the 1999-2004 conference order form
Transplant Immunology 101 — M. Grebenau — March 2, 2004
The Ins and Outs of CPT Codes — P. Kimball, R. Kerman, and W. LeFor — March 16, 2004
An Overview of the Complement System — N. Kramer — March 23, 2004
Recent Advances in Stem Cell Therapy for Autoimmune Disorders— R. Burt — April 6, 2004
Search Strategies: Finding the Best Mismatched Donor — M. Oudshoorn — April 20, 2004
Improving the Performance Improvement Process — B. Parsons — April 27, 2004
Efficient and Effective Assay Validation — L. Osowski and A. Hurst — May 4, 2004
Implementing, Optimizing, Troubleshooting the ELISA Crossmatch — P. Adams — May 18, 2004
Chronic Allograft Rejection: What Is It and Why Is It Such a Problem? — C. Orosz — May 25, 2004
Emerging Molecular Technologies — E. Wang — June 15, 2004
Haplotype-Specific Extraction — J. Dapprich and N. Murphy — June 22, 2004
Transplantation: Unique Challenges in Transfusion Medicine— S. Harris — June 29, 2004
Post Transplant Antibody Testing — E. Reed — July 13, 2004
Demystifying the NMDP — M. Setterholm — July 27, 2004
Antigen Presentation — M. Grebenau — August 3, 2004
Implementing, Optimizing, Troubleshooting the B Cell Crossmatch — C. Murphey — August 10, 2004
Tumor Vaccines: What are They and How are They Made? — F. Marincola — September 21, 2004
Search Strategies II: Case Studies — S. Rosen-Bronson — October 26, 2004
The Role of Alloantibody in Kidney Transplant Deterioration — P. Halloran — November 16, 2004
HLA for Beginners — S. Rosen-Bronson — March 27, 2003
An HLA Nomenclature Update for 2003 — E. Albert — April 3, 2003
Non-Myeloablative Hematopoietic Stem Cell Transplant: Theory and Practice — M. de Lima — April 17, 2003
Donor Search Strategies: A Systematic Approach — L. Edwins and S. Rosen-Bronson — April 24, 2003
DNA for Beginners — C. Hurley — May 8, 2003
Back to the Basics: Understanding, Optimizing, and Troubleshooting AHG-CDC Assays — A. Fuller — May 15, 2003
The ELISPOT Assay: What is it? And How Can It Be Useful in Transplantation — A. Tambur — May 22, 2003
KIR: What are they? How does one KIR “Type”? Their Role in Transplantation Outcome? — K. Hsu — July 17, 2003
Cord Blood as a Source of Stem Cells for Transplantation — L. Baxter-Lowe — July 31, 2003
Transfusion Related Acute Lung Injury (TRALI) — S. Roseff — August 14, 2003
Pretransplant Antibody Testing for Renal Transplantation — H. Gebel — September 18, 2003
HLA Matching Requirements for BMT — M. Fernandez-Vina, V. Turner & S. Rosen-Bronson — September 25, 2003
IvIg Desensitization Protocols: The Laboratories Role — C. Murphy, D. Tyan, & A. Zachary — October 9, 2003
Luminex — J. Jacobson — February 20, 2002
Xenotransplantation — A. J. Tector — April 17, 2002
Ethical Considerations in HSC Transplantation — J. Kahn — May 8, 2002
Importance of HLA Matching in Organ Transplant — H. Gebel — July 24, 2002
VNTR, STR, SNP — S. Rosen-Bronson — August 21, 2002
Clinical Significance of Flow Crossmatching and Antibody Testing — P. Nickerson — September 4, 2002
Immunune Suppression: Behind the Scenes, Part I — R. Bray & H. Gebel — June 13, 2001
Immunune Suppression: Behind the Scenes, Part II — R. Bray & H. Gebel — June 27, 2001
Graft Versus Host Disease — N. Flomenberg — August 15, 2001
Beyond HLA-A, -B and -DR — C. Hurley — January 19, 2000
How to Optimize and Interpret Flowcytometric PRA Assays — R. Bray & H. Gebel — April 14, 1999
March 2, 2004
Transplant Immunology 101
Mark Grebenau, M.D., Ph.D.
Director of Medical Information for Transplant Immunology
Novartis Pharmaceuticals, East Hanover, NJ
This basic lecture will provide an overview of the immune system and the immune response mounted against transplanted cells, tissues and organs. This conference will be particularly useful for
technologists studying for the CHT or CHS certification exams.
March 16, 2004
The Ins and Outs of CPT Codes
Pamela Kimball, Ph.D., Medical College of Virginia, Richmond, VA
Ronald Kerman, Ph.D., University of Texas Medical School, Houston, TX
William LeFor, Ph.D., LifeLink Foundation Inc., Tampa, FL
This advanced level lecture will be of interest to all individuals involved with billing for histocompatibility testing. Participants will learn how existing CPT codes can be successfully and legitimately used to bill
for assays performed in histocompatibility laboratories.
March 23, 2004
An Overview of the Complement System
Norman Kramer, M.D.
MEDIMMGEN Consultants Inc., Burtonsville , MD
This basic level lecture will review the complement system and its role in the immune response. This conference will be especially useful for those new to the field of histocompatibility and those studying
for certification exams.
April 6, 2004
Recent Advances in Stem Cell Therapy for Autoimmune Disorders
Richard Burt, M.D.
Northwestern Memorial Hospital, Chicago , IL
This intermediate level lecture will discuss how stem cell transplantation is being used as a treatment
for an increasing number of autoimmune diseases including lupus, multiple sclerosis, and other debilitating and life threatening diseases.
April 20, 2004
Search Strategies I: Finding the Best Mismatched Donor
Machteld Oudshoorn, Ph.D.
Europdonor Foundation, Leiden , Netherlands
This intermediate level lecture will discuss the various factors that should be taken into consideration when a patient does not have a perfectly matched donor available for an unrelated stem cell transplant. The conference will address a variety of questions including: How many alleles can be mismatched? What search strategies should be used to find the best mismatch? What non-HLA factors such as age
and sex should be considered?
April 27, 2004
Improving the Performance Improvement Process
Barbara Parsons, M.A., M.T.
Johns Hopkins Hospital, Baltimore, MD
This intermediate level lecture will be of interest to anyone interested in performance improvement. Laboratories, donor centers, and transplant centers alike all have accrediting agencies closely
scrutinizing their programs for quality assurance and process improvement. This conference will help participants understand the overall principle of performance improvement and learn how a
well-designed program can make performance improvement an integral part of their institution’s
daily operations.
May 4, 2004
Efficient and Effective Assay Validation
Lori Osowski, M.S., C.H.S.
Ashley Hurst, M.T, C.H.T.
American Red Cross, National HLA Laboratory, Baltimore , MD
This basic lecture will teach participants how to design appropriate validation studies to meet the requirements of ASHI and CLIA for accreditation of new assays being implemented in their laboratory. This conference will be particularly useful for technologists studying for the CHT or CHS certification exams.
May 18, 2004
Implementing, Optimizing and Troubleshooting the ELISA Crossmatch
Patrick Adams, M.S., C.H.S.
Ohio State University Hospital, Columbus, OH
This basic lecture will discuss the principle of how the ELISA crossmatch works as well as how to implement and optimize this assay for use in the histocompatibility laboratory. As more an more laboratories introduce solid phase assays into their routine test repertoires, there is a growing interest
in and need for an ELISA-based crossmatch. This conference will address the pros and cons of the methodology. It will help participants decide if it is right for their laboratory and if it meets the needs
of their transplant program.
May 25, 2004
Chronic Allograft Rejection: What Is It and Why Is It Such a Problem?
Charles Orosz, Ph.D.
Ohio State University Hospital, Columbus, OH
This intermediate level lecture will be of particular interest to individuals working in the area of solid organ transplantation. Participants will learn what is known about the underlying causes of chronic rejection and gain an understanding of why it remains one of the most difficult barriers to long-term
graft survival.
June 15, 2004
Emerging Molecular Technologies
Ena Wang, M.D.
Department of Transfusion Medicine, NIH Clinical Center, Bethesda, MD
This advanced lecture will provide an overview of emerging molecular tools such as real time PCR, microarrays, and pyrosequencing. Participants will learn how these techniques can be useful in the
HLA laboratory and how they are used for studying immune responsiveness and developing new immunotherapies.
June 22, 2004
Haplotype-Specific Extraction (HSE): An Innovative New Technology
Johannes Dapprich, Ph.D., Generation Biotech, LLC, Lawrenceville, NJ
Nancy Murphy, B.A., GenoVision, Inc., West Chester, PA
This intermediate level lecture will discuss an innovative new technology developed by Dr. Dapprich. Haplotype-Specific Extraction, HSE, was designed to physically separate a diploid sample into its
haploid components which can then be separately analyzed by standard DNA typing methods currently used on diploid DNA.
June 29, 2004
Transplantation: Unique Challenges in Transfusion Medicine
Shealynn Harris, M.D.
Transfusion Medicine Service, Department of Pathology
Emory University Hospital, Atlanta, GA
Transplantation patients can present unique problems in transfusion medicine. For example,
complications may include TRALI, GVHD, platelet refractoriness, and confusing RBC phenotypes.
This intermediate level lecture will provide an overview of transplant patient related issues critical to transfusion medicine.
July 13, 2004
Post Transplant Antibody Testing
Elaine Reed, Ph.D.
UCLA Immunogenetics Center, Los Angeles, CA
HLA antibodies can reoccur or occur de novo post transplant. There is increasing evidence that donor specific antibodies play a role in graft rejection and tissue damage. There is also evidence that the detection of donor specific HLA antibody or C4d deposition can provide early warning of rejection. This intermediate level lecture will discuss various assays that can help monitor and predict transplant rejection.
July 27, 2004
Demystifying the NMDP
Michelle Setterholm, M.T.
National Marrow Donor Program, Minneapolis , MN
This basic lecture will discuss the role of the NMDP in facilitating blood and marrow transplantation. Participants will learn about the mission of the NMDP and how it works. They will also learn how their laboratory or transplant program can more effectively work together with the NMDP to expedite the donor search process.
August 3, 2004
Antigen Presentation
Mark Grebenau, M.D., Ph.D.
Director of Medical Information for Transplant Immunology
Novartis Pharmaceuticals, East Hanover, NJ
This intermediate level lecture will describe how antigens are processed and presented to T cells by
HLA
molecules. This conference will be particularly useful for technologists studying for certification exams.
August 10, 2004
Implementing, Optimizing, and Troubleshooting the B Cell Crossmatch
Cathi Murphey, M.T., C.H.S.
Southwest Immunodiagnostics Inc., San Antonio , TX
As evidence increases that the presences of donor specific HLA class II antibody represents an
increased risk to successful transplant outcome, more and more laboratories are routinely performing
a B cell crossmatch prior to transplantation. However, this assay can be difficult to standardize and technically challenging. This intermediate to advanced level lecture will discuss ways to optimize and troubleshoot flowcytometric B cell crossmatches.
September 21, 2004
Tumor Vaccines: What are They and How are They Made?
Francesco Marincola, M.D.
Department of Transfusion Medicine, NIH Clinical Center, Bethesda, MD
Tumor antigen-specific vaccines used for cancer immunotherapy can generate tumor specific CD8 responses detectable in PBMCs and in turn infiltrating lymphocytes. This intermediate level lecture will discuss how tumor vaccines are developed and why they are useful as therapeutic agents for certain types of cancers.
October 26 , 2004
Search Strategies II: Case Studies
Sandra Rosen-Bronson, Ph.D.
Georgetown University Hospita, Washington , DC
This advanced lecture will review a series of BMT case studies which demonstrate how the search strategy principals and tools described in earlier teleconferences can be used to effectively and efficiently identify the optimal donor for a patient.
November 16, 2004
The Role of Alloantibody in Kidney Transplant Deterioration
Philip Halloran, M.D., Ph.D.
Director, Division of Nephrology and Immunology
University of Alberta, Edmonton, Alberta, Canada
This advanced level lecture will discuss how the presence of HLA antibody plays a role in tissue damage and overall deterioration of the transplanted organ. Dr. Halloran will also discuss assays and novel technologies that will enable detection of allograft dysfunction or rejection, monitor responses to
therapy, and predict long-term outcomes.
March 27, 2003
HLA for Beginners
Sandra Rosen-Bronson, Ph.D., Georgetown University Hospital, Washington, DC
This basic lecture will be of particular interest to technologists new to the field of histocompatibility as well as transplant nurses and coordinators interested in learning more about the HLA system and its
role in transplantation.
April 3, 2003
An HLA Nomenclature Update for 2003
Ekkehard Albert, Ph.D.
Laboratory of Immunogenetics, LMU, Munich, Germany
This intermediate level lecture will cover basic concepts of genetic terminology relevant to histocompatibility and immunogenetics. Dr Albert will describe HLA nomenclature and will give an overview of important nomenclature changes and updates recently implemented by the WHO Nomenclature Committee.
April 17, 2003
Non-Myeloablative Hematopoietic Stem Cell Transplant: Theory and Practice
Marcos de Lima, M.D.
Anderson Cancer Center, Houston, TX
This basic lecture will discuss new methods for transplantation that involve the use of non-ablative
doses of chemotherapy or radiation therapy prior to stem cell transplant. The speaker will review the theory behind this relatively new approach to stem cell transplantation.
April 24, 2003
Donor Search Strategies: A Systematic Approach
Linda Edwins, CHS, Clearwater, FL
Sandra Rosen-Bronson, Ph.D., Georgetown University Hospital, Washington, DC
This intermediate to advanced level conference will be of particular interest to transplant coordinators and histocompatibility professionals involved in donor selection for hematopoeitic stem cell transplantation. This lecture will help you develop a systematic stepwise approach to reviewing difficult patient searches. Tools will be provided to help you develop search strategies and sample cases will be reviewed to help you learn how to apply these strategies to a patient search.
May 8, 2003
DNA for Beginners
Carolyn Hurley, Ph.D.
Georgetown University , Washington , DC
This lecture will review basic molecular biology concepts and provide a basic overview of methodologies used for DNA-based HLA typing methods.
May 15, 2003
Back to the Basics: Understanding, Optimizing and Troubleshooting AHG-CDC Assays
Ann Fuller, CHS
University of Utah, Salt Lake City, Utah
This basic lecture will be of particular interest to technologists new to the field of histocompatibility testing. Although there are many new technologies being employed by histocompatibility laboratories, AHG-CDC-based assays are still critical and routinely used tests in most laboratories. This lecture will review the basic concepts and application of anti-human globulin (AHG) augmented lymphocytotoxicity assays. A discussion of assay optimization and troubleshooting will be included.
May 22, 2003
The ELISPOT Assay: What is it? And How Can It Be Useful in Transplantation
Anat Tambur, D.M.D., Ph.D.
Rush Presbyterian St. Luke’s Medical Center, Chicago, IL
This intermediate level lecture will discuss theory, principal and practical applications for the enzyme-linked immuno-spot ELISPOT assay. This assay first described two decades ago as a useful assay for
the detection of specific immune responses on a single cell level is being used more and more as a method for post-transplant monitoring.
July 17, 2003
KIR: What are they? How does one KIR “Type”? How Might KIR Play an Important Role in
Transplantation Outcome?
Katharine Hsu, Ph.D.,
Memorial Sloan Kettering Cancer Center , New York , NY
This intermediate level lecture will describe killer cell immunoglobulin-like receptors (KIR) and how
they may be important in transplantation as well as methods currently used for KIR typing.
July 31, 2003
Cord Blood as a Source of Stem Cells for Transplantation
Lee Ann Baxter-Lowe, Ph.D.
University of California, San Francisco, San Francisco, CA
This lecture will be of general interest to all individuals interested in hematopoeitic stem cell transplantation. Dr. Baxter-Lowe will discuss issues concerning cord blood banking as well as review current and emerging data concerning cord blood transplantation.
August 14, 2003
Transfusion Related Acute Lung Injury (TRALI)
Susan Roseff, M.D.
Virginia Commonwealth University School of Medicine, Richmond, VA
This basic lecture will discuss TRALI, an often misdiagnosed, life threatening pulmonary syndrome thought to be caused by HLA specific antibodies of donor origin present in plasma containing blood products.
September 18, 2003
Pretransplant Antibody Testing for Renal Transplantation: A Review of Current Data
Howard Gebel, Ph.D.
Emory University, Atlanta, GA
This lecture will be of general interest to all individuals interested in renal transplantaion. Dr. Gebel
will provide an overview of current data concerning the relevance of HLA antibody testing in renal transplantation and the significance of preformed HLA specific antibody to clinical outcome.
September 25, 2003
HLA Matching Requirements for Hematopoeitic Stem Cell Transplantation: A Review of Current Data
Marcelo Fernandez-Vina, Ph.D., Georgetown University, Washington, DC
Victoria Turner, Ph.D., St. Jude Hospital, Memphis, TN
Sandra Rosen-Bronson, Ph.D., Georgetown University Hospital, Washington, DC
This intermediate level conference will be of particular interest to transplant coordinators and histocompatibility professionals involved in hematopoeitic stem cell transplantation. The lecture will provide an overview of current and emerging data concerning matching requirements for HSC transplantation. The discussion will include evolving data concerning other donor variables such as age and sex matching versus level of HLA match and current information concerning potential differential matching requirements for adults versus pediatric patients.
October 9, 2003
IvIg Desensitization Protocols: The Laboratories Role
Cathi Murphy, CHS, Southwest Immunodiagnostics, San Antonio, TX
Dolly Tyan, Ph.D., Cedars-Sinai Medical Center, Los Angeles, CA
Andrea Zachary, Ph.D., Johns Hopkins University, Baltimore, MD
This basic to intermediate level lecture will cover the different laboratory methods used to evaluate
and support IvIg desensitization protocols for transplant patients with donor specific HLA antibodies. The lecture will include description of CDC, ELISA, and flowcytometric methods.
February 20, 2002
Luminex: An Innovative New Technology that Tests Multiple Analytes in a Single Reaction
James Jacobson, Ph.D.
Luminex Corporation, Austin, TX
Learn about the Luminex technology that uses color-coded microspheres and reporter molecules for a variety of applications including SSO-based HLA typing, antibody detection, SNP typing and more.
April 17, 2002
Xenotransplantation: The Promise and the Problems
A. Joseph Tector, M.D.
Indiana University, Department of Surgery, Indianapolis, IN
Learn about the therapeutic potential, immunologic hurdles and infectious risks of xenotransplantation.
May 8, 2002
Ethical Considerations in Hematopoietic Stem Cell Transplantation
Jeffrey Kahn, Ph.D., M.P.H.
University of Minnesota, Center for Bioethics, Minneapolis, MN
Learn about ethical issues and controversies connected with HSC donation and transplantation.
July 24, 2002
Histocompatibility Testing For Solid Organ Transplantation: Is it Still Important?
Howard Gebel, Ph.D.
Emory University, Atlanta, GA
Learn about the pros and cons of the current debates concerning the importance of HLA matching and histocompatibility testing in organ transplantation.
August 21, 2002
VNTR, STR, SNP: What are They?
How are They Useful in Transplantation and Immunogenetics?
Sandra Rosen-Bronson, Ph.D.
Georgetown University Hospital, Washington, DC
Learn about these genetic markers and how they are used to monitor engrafment, detect chimerisms and locate desease genes.
September 4, 2002
The Clinical Significance of Flowcytometric Crossmatching and Antibody Testing
Peter Nickerson, M.D.
Canadian Blood Services, Winnipeg, Manitoba, Canada
Learn about the continued clinical significance of HLA antibidies detected by flowcytometric methods in the era of modern immunosuppresssion.
June 13, 2001
Immunune Suppression: Behind the Scenes, Part I
Robert Bray, Ph.D.
Emory University, Atlanta, GA
Howard Gebel, Ph.D.
Lousiana State University, Shreveport, LA
Learn about T cell activation pathways and how they are affected by current immunosuppressive
agents.
June 27, 2001
Immunune Suppression: Behind the Scenes, Part II
Robert Bray, Ph.D., Emory University, Atlanta, GA
Howard Gebel, Ph.D., Lousiana State University, Shreveport, LA
Learn about T cell activation pathways and how they are affected by current immunosuppressive
agents.
August 15, 2001
Graft Versus Host Disease
Neal Flomenberg, M.D.
Thomas Jefferson University, Philadelphia, PA
Learn about graft versus host phenomena, current approaches for treatment and when and how it can
be beneficial.
January 19, 2000
Beyond HLA-A, -B and -DR
Carolyn Hurley, Ph.D.
Georgetown University Medical Center, Washington, DC
Learn about the role of other non-HLA molecules in transplantation.
April 14, 1999
How to Optimize and Interpret Flowcytometric PRA Assays
Robert Bray, Ph.D.
Emory University, Atlanta, GA
Howard Gebel, Ph.D.
Lousiana State University, Shreveport, LA
Learn how to optimize and interpret various flowcytometric assays for the detection of HLA antibodies. This lecture covers both cell-based and solid-phased techniques.
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